Journal: Cells

Article Title: Progranulin Is a Survival Factor for Axotomized Retinal Ganglion Cells in Adult Mice

doi: 10.3390/cells15110988

Figure Lengend Snippet: BRN-3A-labeled RGCs in flat-mounted retinas. Retinas treated with PGRN-NSCs ( a – d ) or control-NSCs ( e – h ) 14 ( a , e ), 28 ( b , f ), 56 ( c , g ), and 112 ( d , h ) days after an intraorbital optic nerve crush. PGRN-treated retinas contained significantly higher numbers of surviving RGCs than control retinas at all post-lesion time points. Abbreviations: BRN-3A, brain-specific homeobox/POU domain protein 3A; DAPI, 4′,6-di-amidino-2-phenylindole; dpl, days post lesion; NSCs, neural stem cells; PGRN, progranulin; RGCs, retinal ganglion cells. Scale bar: 50 μm.

Article Snippet: To visualize the surviving RGCs, retinal flatmounts were incubated overnight with rabbit antibodies directed against brain-specific homeobox/POU protein 3A (BRN-3A; 1:1000; Synaptic Systems, Göttingen, Germany), followed by Cy3-conjugated donkey anti-rabbit secondary antibodies (1:200; Dianova) and DAPI.

Techniques: Labeling, Control

Journal: Contrast Media & Molecular Imaging

Article Title: Key Parameters on the Microwave Assisted Synthesis of Magnetic Nanoparticles for MRI Contrast Agents

doi: 10.1155/2017/8902424

Figure Lengend Snippet: MRI T 1 and T 2 relaxation times as a function of the Fe concentration for DMSA coated magnetite nanoparticle suspensions obtained by microwave (MW) and thermal decomposition (TD) using different solvents.

Article Snippet: Finally, MRI relaxometric properties were investigated by measuring the longitudinal ( T 1 ) (sequence t1_ir_mb) and transversal ( T 2 ) (sequence t2_ir_mb) protons relaxation times at different dilutions between 0 and 0.07 mM of Fe in a MINISPEC MQ60 (Bruker) at 37°C and a magnetic field of 1.5 T. The sequences used are original from Bruker.

Techniques: Concentration Assay

Journal:

Article Title: Heteroduplex Panel Analysis, a Novel Method for Genetic Identification of Aspergillus Section Flavi Strains

doi: 10.1128/AEM.67.9.4084-4090.2001

Figure Lengend Snippet: HPA panels of Aspergillus Section Flavi strains obtained by using ITS-region DNAs amplified by PCR. The left panels of reference strains P-39-1, IFO4082, A0754, and ATCC 15546 are the standard panels of F-1, P-1, T-1, and N-1, respectively. On the right are the test panels of 20 strains that are indicated above the panels. F-2, N-2, N-3, and TN-1 are additional new panels of heteroduplex types. Heteroduplex bands in lanes f, p, t, and n were formed with reference strains of A. flavus, A. parasiticus, A. tamarii, and A. nomius, respectively.

Article Snippet: Except for type TN-1, intraspecific diversity (1 to 3 bp) did not display any heteroduplex bands with mobility shifts (lane f of F-2 and lanes n of N-2 to N-3 in Fig. ), while other interspecific heteroduplexes (lanes p, t, and n of F-2 and lanes f, p, and t of N-2 and N-3 in Fig. ) displayed mobility shifts apparently different from those of the corresponding standard panels. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Type a Nucleotide diversity (no. of bases) from: F-1 (595) b F-2 (594) P-1 (595) T-1 (598) N-1 (598) N-2 (598) N-3 (598) TN-1 (597) F-1 0 F-2 1 0 P-1 8 9 0 T-1 13 14 9 0 N-1 20 21 14 13 0 N-2 19 20 13 12 2 0 N-3 19 20 13 12 1 3 0 TN-1 14 15 8 5 8 8 7 0 Open in a separate window a F-1, A. flavus P-39-1 and A. oryzae IFO 30113; F-2, A. oryzae IFO 5375; P-1, A. parasiticus IFO 4082 and A. sojae IFO 4200; T-1, A. tamarii A0754; N-1, A. nomius ATCC 15546; N-2, A. nomius NRRL 6552; N-3, A. nomius IMI 358751; TN-1, A. nomius IMI 358749. b The numbers within parentheses indicate the sizes (base pairs) of ITS region DNAs amplified by PCR.

Techniques: Amplification

Journal:

Article Title: Heteroduplex Panel Analysis, a Novel Method for Genetic Identification of Aspergillus Section Flavi Strains

doi: 10.1128/AEM.67.9.4084-4090.2001

Figure Lengend Snippet: Nucleotide sequence alignments for ITS1 (A) and ITS2 (B) of rRNA genes from Aspergillus Section Flavi strains. F-1, A. flavus P-39-1; F-2, A. oryzae IFO 5375; P-1, A. parasiticus IFO 4082; T-1, A. tamarii A0754; N-1, A. nomius ATCC 15546; N-2, A. nomius NRRL 6552; N-3, A. nomius IMI 358751; TN-1, A. nomius IMI 358749. Dots indicate identity to the sequence of F-1. Dashes indicate alignment gaps (insertion or deletion differences). Boxes A to E indicate variable regions.

Article Snippet: Except for type TN-1, intraspecific diversity (1 to 3 bp) did not display any heteroduplex bands with mobility shifts (lane f of F-2 and lanes n of N-2 to N-3 in Fig. ), while other interspecific heteroduplexes (lanes p, t, and n of F-2 and lanes f, p, and t of N-2 and N-3 in Fig. ) displayed mobility shifts apparently different from those of the corresponding standard panels. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Type a Nucleotide diversity (no. of bases) from: F-1 (595) b F-2 (594) P-1 (595) T-1 (598) N-1 (598) N-2 (598) N-3 (598) TN-1 (597) F-1 0 F-2 1 0 P-1 8 9 0 T-1 13 14 9 0 N-1 20 21 14 13 0 N-2 19 20 13 12 2 0 N-3 19 20 13 12 1 3 0 TN-1 14 15 8 5 8 8 7 0 Open in a separate window a F-1, A. flavus P-39-1 and A. oryzae IFO 30113; F-2, A. oryzae IFO 5375; P-1, A. parasiticus IFO 4082 and A. sojae IFO 4200; T-1, A. tamarii A0754; N-1, A. nomius ATCC 15546; N-2, A. nomius NRRL 6552; N-3, A. nomius IMI 358751; TN-1, A. nomius IMI 358749. b The numbers within parentheses indicate the sizes (base pairs) of ITS region DNAs amplified by PCR.

Techniques: Sequencing

Journal: Ecology and Evolution

Article Title: Are geometric morphometric analyses replicable? Evaluating landmark measurement error and its impact on extant and fossil Microtus classification

doi: 10.1002/ece3.6063

Figure Lengend Snippet: Pairwise analysis of landmark datasets comparing Procrustes ANOVA residual R 2 percentages (ProcANOVA R 2 [%]) and repeatability among datasets, absolute differences among comparisons in cross‐validated linear discriminant analysis predicted group membership error (PGM Error Change [%]), and differences among comparisons in the percent of predicted group membership changes among individual Project 23 fossils of unknown species affinity (Fossil PGM Change [%])

Article Snippet: Dinolite_NoTilt_ EO _T1–Dinolite_NoTilt_ NO _T1 , Interobserver , 0.21 , 31.01 , 20.6 , 32.3.

Techniques: Imaging